اثر پیش تیمارهای دمایی و محیط کشت بر کال‌زایی و باززایی تلاقی‌های مرکب ارقام پر محصول برنج با لاین قائم

In the present study, the effect of temperature pretreatment (T1=4 and T2= 8 °C) for 10 days on anthers of six different genetic compositions of Ghaem with other high-yielding lines of rice have been studied. For callus induction, three modified culture media of N6 (N1, N2 and N3) and MS culture medium was used for regeneration. results of statistical analysis showed that there was significant difference (P<0.01) among genotypes, modified media of N6, temperature pretreatment and their interactions on callus induction. The genetic composition of(Ghaem /fajr // Ghaem /Domsiah)  was identified as the most suitable genotype regarding to the callus induction (18.2%, at 8°C cold pretreatment). Among modified media of N6, medium the N1 medium (N6+ 0.5 mg/L-1 2,4-D + 2.5 mg/L-1 NAA + 0.5 mg/L-1 Kin + 20 g/L-1 maltose and 30 g/L-1 sucrose) was evaluated as the best culture medium. Among pre-treated callus at 8 °C, the highest regeneration was related to genetic composition of­ (Ghaem /Nemat // Ghaem /shastak)  with 23.3% regeneration (albino plant)successes. In current study 100% of regenerated plants were albino and the temperature of 8 °C pretreatment for 10 days was the best for callus induction and regeneration of these genetic compositions.